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KMID : 0380620210530020195
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Korean Journal of Food Science and Technology
2021 Volume.53 No. 2 p.195 ~ p.203
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Optimization of cultivation conditions for pullulan production from Aureobasidium pullulans MR by response surface methodology
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Jo Hye-Mi
Kim Ye-Jin
Yoo Sang-Ho
Kim Chang-Mu
Kim Gye-Won
Park Cheon-Seok
Park Cheon-Seok
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Abstract
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Aureobasidium pullulans, a black yeast, produces pullulan, a linear α-glucan composed of maltotriose repeating units linked by α(1→6)-glycosidic linkages. Pullulan can be widely used in food, cosmetic, and biotechnology industries.
In this study, we isolated eight strains of A. pullulans from Forsythia koreana, Magnolia kobus DC., Spiraea prunifolia var. simpliciflora, Cornus officinalis, Cerasus, and Hippophae rhamnoides. Among them, A. pullulans MR was selected as the best pullulan producer. The effects of a carbon source, a nitrogen source, and pH on pullulan production were examined. The optimal cultivation conditions for pullulan production by A. pullulans MR were determined by response surface methodology as 15% sucrose, 0.4% soy peptone, and an initial pH of 7 at 26oC. Under these conditions, the predicted pullulan production was 47.6 g/L, which was very close to the experimental data (48.9 g/L).
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KEYWORD
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Aureobasidium pullulans, pullulan, optimization, response surface methodology, yeast
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